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The Basics of DNA Purification

It is important to have a high-quality DNA that is free of contaminants such as http://www.mpsciences.com/2021/04/23/dna-purification-processes-for-different-applications/ protein, debris, and RNA before performing the PCR or cloning process, or DNA sequencing. Purifying DNA is also known as DNA Isolation, and is an essential step in molecular biology. In this article, you’ll learn the basics of DNA purification and how you can optimize your DNA extraction techniques to get better results.

The first step of the DNA purification procedure is to prepare a solution containing an emulsion of water and alkaline buffer. This buffer makes DNA soluble, which means it can be easily separated from other components of the sample. After the DNA is placed in an alkaline solution and a water solution, it is then treated with detergents and salts that break down cell membranes and nuclei. This lets the DNA out. RNase can be added to the sample in order to remove any DNA that has been contaminated.

DNA is separated from other cellular components like proteins and lipids with the help of organic solvents such as chloroform and phenol. Once the DNA has been separated from proteins and lipids, it can be extracted using ethanol or isopropyl alcohol (rubbing alcohol).

The quality of the DNA may be assessed by spectrophotometry or gel electrophoresis. A high-quality DNA sample should have a ratio of absorbance at 260 nm to 280 nm of 1.8. A low ratio could signal a problem with the protein binding process or carryover of salt from wash or bind buffers.